Jorge D. García-García

This study improves the aerotolerance and activity of sulfide-dependent THI4 enzymes through directed evolution in yeast, identifying beneficial mutations that enhance their performance under aerobic conditions.

The paper describes a method to improve enzymes for plant applications using continuous directed evolution in yeast, demonstrating its effectiveness with a bacterial thiamin synthesis enzyme.

The paper identifies aerotolerant, multiple-turnover THI4 thiazole synthases in aerobic prokaryotes and shows they use cobalt as a cofactor instead of iron.

The paper discusses two systems for continuous directed evolution of enzymes and tests their adaptation for plant enzyme engineering.