Bryan J. Leong

This study uses directed evolution to extend the lifespan of an Arabidopsis enzyme by increasing its abundance and improving its resistance to a toxic intermediate.

This study uses continuous directed evolution to engineer feedback-resistant Arabidopsis arogenate dehydratase in a microbial platform, achieving resistance comparable to a known variant.

The paper describes a method to improve enzymes for plant applications using continuous directed evolution in yeast, demonstrating its effectiveness with a bacterial thiamin synthesis enzyme.

The paper identifies aerotolerant, multiple-turnover THI4 thiazole synthases in aerobic prokaryotes and shows they use cobalt as a cofactor instead of iron.

The paper investigates the evolution of a hyper-diverse acylsugar biosynthetic pathway in the Solanaceae family using multi-omic approaches, revealing mechanisms of molecular innovation over 100 million years.