Zbyszek Otwinowski

The paper identifies limitations in current cryo-EM data deposition practices and proposes an alternative approach to better preserve information and support validation.

The paper reports a cryo-EM structure of detergent-bound Vibrio cholerae MakA, revealing its conformational changes and role in pore assembly.

The paper determines six structural states of PilU, a retraction ATPase, revealing its homohexameric assembly and conformational flexibility, which may explain its weak ATPase activity and dependence on PilT.

This paper introduces a method to validate 3D cryoEM reconstructions by separating scattering contributions into real and imaginary components to determine handedness and assess reconstruction quality.

The paper presents cryo-EM structures of the TolQ-TolR complex from Acinetobacter baumannii, revealing structural changes related to PMF energy transfer and cell envelope organization.

The paper develops DeepSAV, a deep-learning model to predict the pathogenicity of single-amino acid variations, and uses it to assess mutation severity across genes, identifying intolerant and tolerant genes for different disease categories.