Michelle A. Digman

The paper introduces an open platform for multiplexed bioluminescence and fluorescence microscopy, including tools for experiment design, microscope upgrades, and data analysis.

This study shows that ASXL1 mutations promote cancer by unleashing phase separation, which enhances gene activation and leukemogenesis.

ESPRESSO provides high-dimensional spatiotemporal phenotyping of organelles using fluorescent labeling and advanced microscopy, enabling analysis of cell states, stress response, and immune cell polarization.

This paper introduces a Bayesian S-FLIM framework that learns spectra and lifetimes photon-by-photon, enabling efficient deconvolution of up to 9 fluorophore species with overlapping spectra.

This study examines lipid polarity and droplet changes in hippocampal neurons across aging and Alzheimer's disease models, finding altered lipid dynamics in AD-transgenic mice.

The paper uses a multimodal imaging approach to study breast cancer cell invasion in 3D spheroids, quantifying changes in metabolism, fatty acid storage, and collagen organization over time.

This study develops bioluminescent reporters to monitor macrophage polarization in real-time with single-cell resolution in 2D and 3D environments.

The paper uses phasor-FLIM to assess pre-implantation embryo quality by analyzing fluorescence lifetime, achieving 86% accuracy in identifying healthy embryos.