Jennifer A. Prescher

A miniature bioluminescent platform for imaging structured RNAs

This paper develops a miniaturized bioluminescent platform for imaging structured RNAs in live cells, achieving a 3-fold reduction in tag size compared to existing methods.

An open, integrated platform for multiplexed bioluminescence microscopy

The paper introduces an open platform for multiplexed bioluminescence and fluorescence microscopy, including tools for experiment design, microscope upgrades, and data analysis.

Continuous hypermutation and evolution of luciferase variants

This paper introduces a streamlined platform for luciferase engineering using continuous hypermutation, resulting in improved light output variants without manual library generation.

ESPRESSO: Spatiotemporal omics based on organelle phenotyping

ESPRESSO provides high-dimensional spatiotemporal phenotyping of organelles using fluorescent labeling and advanced microscopy, enabling analysis of cell states, stress response, and immune cell polarization.

Bioorthogonal cyclopropenones for investigating RNA structure

This paper develops bioorthogonal cyclopropenones to crosslink RNA and study its native structures in cells.

Monitoring macrophage polarization with gene expression reporters and bioluminescence phasor analysis

This study develops bioluminescent reporters to monitor macrophage polarization in real-time with single-cell resolution in 2D and 3D environments.

A modular platform for bioluminescent RNA tracking

This paper introduces a bioluminescent platform for tracking RNA in vivo, demonstrating single-copy detection and robust imaging in cells and live animals.