Lorenzo Scipioni

The paper introduces an open platform for multiplexed bioluminescence and fluorescence microscopy, including tools for experiment design, microscope upgrades, and data analysis.

ESPRESSO provides high-dimensional spatiotemporal phenotyping of organelles using fluorescent labeling and advanced microscopy, enabling analysis of cell states, stress response, and immune cell polarization.

This paper introduces a Bayesian S-FLIM framework that learns spectra and lifetimes photon-by-photon, enabling efficient deconvolution of up to 9 fluorophore species with overlapping spectra.

The paper uses a multimodal imaging approach to study breast cancer cell invasion in 3D spheroids, quantifying changes in metabolism, fatty acid storage, and collagen organization over time.

This study develops bioluminescent reporters to monitor macrophage polarization in real-time with single-cell resolution in 2D and 3D environments.

The paper introduces an organelle phenotyping approach using live-cell microscopy to identify C1q as a regulator of microglial function, showing its role in fatty acid storage and mitochondrial membrane potential.