Joshua A. Pixley

PANCS-Inhibitors rapidly selects molecules that directly disrupt pre-formed protein-protein interactions, demonstrating effectiveness against three oncogenic PPIs.

PANCS-spec-Binders rapidly identifies binders with defined specificity by combining selection and counterselection to target specific isoforms or epitopes.

The paper identifies target-specific fitness landscapes in protein-protein interactions and develops a supervised contrastive learning approach to predict binders.

This paper introduces PANCS-binders, an in vivo selection method for discovering protein binders, and demonstrates its ability to screen billions of variants against multiple targets in two days.