Pao‐Wan Lee

A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications

The paper develops a T7 RNA polymerase regulatory toolbox for cell-free systems, enabling gene circuit design and biosensing of various biomolecules with high sensitivity.

De novo design of phosphorylation-induced protein switches for synthetic signaling in cells

This paper designs phosphorylation-induced protein switches that control transcription in cells, demonstrating their functionality in synthetic signaling.

Targeting protein-ligand neosurfaces using a generalizable deep learning approach

This paper designs proteins that target neosurfaces from protein-ligand complexes using a deep learning approach, validated experimentally against three drug-bound protein complexes.