Development and application of nbLIBRA-seq for high-throughput discovery of antigen-specific nanobodies
Sabina E W Leonard, Perry T. Wasdin, Katherine Webb, Parastoo Amlashi, Chloe Renken, Jeffrey C. Rathmell, Benjamin W. Spiller, Brian E. Wadzinski, James E. Crowe, Ivelin S. Georgiev, Kelsey Voss
This paper develops and validates nbLIBRA-seq, a method for high-throughput antigen-specific nanobody discovery in immunized alpacas, demonstrating its effectiveness in identifying nanobodies against CD71 and viral antigens.
Cell Reports Methods · Sep 1, 2026Peer-reviewedImmunology