James E. Crowe

Dengue virus 2 lacking N153 glycosylation displayed enhanced recognition by neutralizing antibodies

The paper shows a dengue virus mutant lacking N153 glycosylation is more susceptible to neutralizing antibodies, as evidenced by increased Fab occupancy and slower dissociation rates.

Development and application of nbLIBRA-seq for high-throughput discovery of antigen-specific nanobodies

This paper develops and validates nbLIBRA-seq, a method for high-throughput antigen-specific nanobody discovery in immunized alpacas, demonstrating its effectiveness in identifying nanobodies against CD71 and viral antigens.

Convergent IGHV3-53/3-66 antibodies elicited by Omicron BA.1 infection broadly neutralize emerging SARS-CoV-2 variants

The paper investigates how IGHV3-53/3-66 antibodies from Omicron BA.1 infection neutralize emerging SARS-CoV-2 variants through structural and mutational analysis.

ACE-2-like Enzymatic Activity in Anti-SARS-CoV-2 Spike Protein Monoclonal Antibodies

This study identifies monoclonal antibodies with ACE2-like catalytic activity that may contribute to post-acute sequelae of COVID-19.

A potently neutralizing and protective human antibody targeting antigenic site V on RSV and hMPV fusion glycoprotein

The paper identifies a human antibody that neutralizes RSV and hMPV by targeting a novel epitope spanning multiple antigenic sites, showing cross-protection in a mouse model.

Glycan-reactive antibodies isolated from human HIV-1 vaccine trial participants show broad pathogen cross-reactivity

The paper isolates glycan-reactive antibodies from HIV-1 vaccine trial participants and finds they neutralize diverse HIV strains and show broad cross-reactivity against other pathogens.

Human coronavirus HKU1 neutralization by glycan receptor mimicry

This study identifies a monoclonal antibody that neutralizes HCoV-HKU1 by mimicking sialic acid binding to block receptor engagement.

Mouse Adapted Omicron BA.5 Induces A Fibrotic Lung Disease Phenotype in BALB/c Mice

This paper develops a mouse model of SARS-CoV-2 BA.5 infection that induces fibrotic lung disease and tertiary lymphoid structures, enabling study of acute and post-acute sequelae of COVID-19.

Cell-based high-content approach for SARS-CoV-2 neutralization identifies unique monoclonal antibodies and PI3K pathway inhibitors.

This study uses high-throughput screens in human cells with authentic SARS-CoV-2 virus to identify neutralizing monoclonal antibodies and kinase inhibitors with minimal host toxicity.

Functional and antigenic landscape of the Nipah virus receptor binding protein

This study uses deep mutational scanning to analyze how mutations in the Nipah virus receptor-binding protein affect function, receptor binding, and antibody escape, revealing constrained regions and limited antigenic variation in natural strains.

Structural Mapping of Polyclonal IgG Responses to HA After Influenza Virus Vaccination or Infection

The paper uses nsEMPEM to analyze polyclonal IgG responses to HA in vaccinated and infected individuals, showing differences in epitope targeting between H1 and H3 subtypes.

Use of equine H3N8 hemagglutinin as a broadly protective influenza vaccine immunogen

This study shows that equine H3N8 vaccine induces broad protection against influenza in multiple animal models, including neutralizing antibodies against diverse viral strains.

Protective threshold of a potent neutralizing Zika virus monoclonal antibody in rhesus macaques

This study shows that ZIKV-117, a neutralizing monoclonal antibody, provides complete protection against ZIKV challenge in rhesus macaques at a low dose.

Discovery and Characterization of a Pan-betacoronavirus S2-binding antibody

The paper identifies a pan-betacoronavirus antibody that binds the S2 subunit of spike proteins and induces Fc-dependent antiviral responses, offering insights into non-neutralizing antibody protection.

Rapid, raw-read reference and identification (R4IDs): A flexible platform for rapid generic species ID using long-read sequencing technology.

This paper introduces R4IDs, a method using long-read sequencing to rapidly generate reference datasets for species identification, validated with plant species and simulations.