Kalyan Shobhana

Single-cell RNA and BCR sequencing of infected mouse spleens shows class switching overlaps clonal expansion, GC-seeding clones also form plasmablasts, and SHM proceeds at constant rate.

This study presents cryo-EM structures of mycobacterial Ku (mKu) in complex with DNA, revealing its role in NHEJ synapsis and identifying key residues for DNA binding and synaptic assembly.