Giuseppe Dall’Agnese

The paper investigates how active RNA synthesis influences nuclear condensate patterning by studying nucleolar fibrillar centers and shows that RNA synthesis affects their size, number, and spacing.

This study creates an endogenous fluorescent APE1 cell line to show that APE1 accumulates in nucleoli via RNA G-quadruplex structures and lysine residues, linking phase separation to its functional switch between rRNA processing and DNA repair.