Rithu K. Pattali

CRISPRtune is a programmable tool that tunes transcriptional repression by fusing MeCP2 to dCas9, enabling intermediate gene expression control in human cells.

This paper demonstrates T cells can deliver genome editing machinery to target cells via programmable intercellular interactions.

LEMONmethyl-seq profiles DNA methylation dynamics in mammalian cells, revealing CRISPR-induced changes and endogenous patterns, including non-canonical CpH methylation.

The paper develops a toolkit of chromatin regulators with cross-kingdom functionality for programmable transcriptional repression and activation in eukaryotic cells.

This paper introduces RENDER, a method for transient delivery of CRISPR-based epigenome editors as ribonucleoproteins to modulate gene expression in human cells, achieving durable epigenetic silencing.

This paper describes a protocol for CRISPRoff epigenetic editing to silence genes in human cells without DNA breaks, showing heritable transcriptional repression.