Thomas Burgold

This paper demonstrates a method using CD117-blocking antibody and epitope-engineered HSPCs to treat β-thalassemia without myeloablative conditioning.

The paper introduces a next-generation dual guide CRISPR system for efficient genetic interaction screening, achieving low undesired guide pairs and balanced expression, and demonstrates its application in colorectal cancer cells.

ONE-STEP tagging enables rapid, efficient, and scalable protein tagging in diverse cell types through CRISPR-Cas9 and Bxb1 integrase, with high integration efficiency and multiplexing capabilities.

This study compares NuRD's role in human and mouse pluripotent stem cells, finding that NuRD is required for transcriptional responses to differentiation signals in both, but with distinct functional differences between species.

The paper shows that MTA proteins in ES cell NuRD complex are functionally redundant, and NuRD is essential for maintaining differentiation trajectory during early lineage commitment.