Author

Michael Timme

Osnabrück UniversityORCID 0009-0007-4255-4270Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell signaling & trafficking· 2 Approach:experimental assay· 2 Research Focus:protein folding & dynamics· 2 Approach:biophysical modeling· 1 Research Focus:cell type annotation· 1 Approach:statistical model· 1

Frequent Co-Authors

Joost C. M. HolthuisiD· 3 shared Manuel N. MeloiD· 3 shared Britta Fiedler· 1 shared Chelsea M. BrowniD· 1 shared D. G. HassaniD· 1 shared Isabelle Watrinet· 1 shared Jacob PiehleriD· 1 shared Milena WessingiD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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3 shared papersBiophysics

Joost C. M. HolthuisORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

BiophysicsbioRxivPreprintApr 28, 2026

Ceramides disrupt hexokinase HKI-VDAC complex assembly and modulate VDAC tilting

Michael Timme, Joost C. M. Holthuis, Manuel N. Melo

This study shows ceramides disrupt VDAC-HKI complex assembly by modulating VDAC tilt and membrane thinning, revealing a mechanism for ceramide-induced apoptosis.

1 citation

biophysical modelingprotein folding & dynamics

𝕏 PostBluesky

BiophysicsbioRxivPreprintNov 3, 2025

Key determinants of VDAC-hexokinase I complex assembly revealed by a minimal vesicle-based interaction assay

Milena Wessing, Isabelle Watrinet, Michael Timme…·Joost C. M. Holthuis

The paper develops an in vitro assay to study HKI-VDAC1 complex assembly and shows the critical role of a bilayer-facing glutamate in pH-sensitive binding.

DOI 10.1101/2025.11.03.686449

experimental assaycell signaling & trafficking

𝕏 PostBluesky

BiochemistryCommunications BiologyPeer-reviewedFeb 10, 2025

Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2

Sebastian Bieker, Michael Timme, Nils Woge…·Joost C. M. Holthuis

This study identifies a charged glutamate on VDAC1 and VDAC2 that interacts with HKI's N-terminal helix, revealing structural and mechanistic insights into their complex formation.

33 citationstop 1%

experimental assaystatistical model

𝕏 PostBluesky