Jacob Piehler

Cholesterol transport cycle of human ABCA2

This study reports cryo-EM structures of human ABCA2, revealing its cholesterol transport mechanism and novel conformational changes.

Structural Rewiring of IL-7R Dimerization by an Oncogenic Transmembrane Mutation Can Be Reversed by Rational Design

This study identifies an oncogenic TMD mutation in IL-7R that causes ligand-independent signaling and shows that rationally designed transmembrane helices can reverse this effect.

Metal-induced energy transfer uncovers activation-induced axial reorganization of signaling complexes inside cells

This study uses metal-induced energy transfer to reveal that cytokine receptor intracellular domains compact upon activation.

A Versatile Toolbox for Nanoscale Interrogation of Multiprotein Assemblies inside Living Cells

This paper introduces a nanopatterning toolbox to study multiprotein assemblies in live cells, demonstrating its application to the myddosome complex and revealing nanoscale organization.

Long-term single molecule localization microscopy uncovers dynamic co-assembly of Lrp6 and Ror2 into Wnt-signalosomes

This study uses single-molecule tracking to show that Lrp6 and Ror2 co-assemble into hybrid Wnt signalosomes dynamically.

Ultrastructural organization and dynamics of TIRAP filaments

This study determines the structure of TIRAP filaments and identifies the role of the N-terminal PBM in regulating their assembly and function at the plasma membrane.

Key determinants of VDAC-hexokinase I complex assembly revealed by a minimal vesicle-based interaction assay

The paper develops an in vitro assay to study HKI-VDAC1 complex assembly and shows the critical role of a bilayer-facing glutamate in pH-sensitive binding.

Design of facilitated dissociation enables control over cytokine signaling duration

The paper designs proteins with controlled dissociation rates by introducing strained excited states, enabling rapid cytokine receptor dissociation.

Live-cell magnetic micromanipulation of recycling endosomes reveals their direct effect on actin-based protrusions to promote invasive migration

The paper uses magnetic micromanipulation to show Rab25 vesicles directly drive F-actin protrusions, enhancing cancer cell invasive migration.

Biofunctional coating of synthetic magnetic nanoparticles enables magnetogenetic control of protein functions inside cells

Mechanism of receptor assembly via the pleiotropic adipokine Leptin

This study reveals the structure and mechanism of Leptin-mediated LEP-R assembly, showing a 3:3 stoichiometry and highlighting the role of Leptin restructuring in activating signaling.

Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses in autoimmune disorders

The paper investigates how differential STAT binding to receptor phospho-Tyr motifs influences cytokine responses, showing altered dynamics in autoimmune disorders like SLE.