Joseph Clarke

This study maps the NFκB pathway in T-cells using CRISPR screens and identifies novel regulators that enhance cytotoxicity and cytokine production.

The paper shows that T-cell activation is initiated by partial CD45 exclusion at sub-micron contacts, leading to strong activation and cytotoxicity through small signaling changes.

This study uses a magnetic particle-based platform to mechanically modulate T-cell receptors, showing that NFAT pathway signaling accumulation is required for CD4+ T cell activation.

The paper uses bulk RNA-seq and single-cell RNA-seq data to study T-cell expansion and identifies FOSL1 as a transcription factor that promotes an effector phenotype, enhancing cancer-cell killing.