bioRxivBiophysicsPreprint
Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting
R. Van den Eynde, F. Hertel, S. Abakumov, B. Krajnik, S. Hugelier, A. Auer, J. Hellmeier, T. Schlichthaerle, R. M. Grattan, D. S. Lidke, R. Jungmann, M. Leutenegger, W. Vandenberg, P. Dedecker
Abstract
We present a way to encode more information in fluorescence imaging by splitting the emission into copies of the original point-spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the Circulator, an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.
- assay: fluorescence / live-cell microscopy
- research focus: imputation
- research focus: dimensionality reduction
- approach: microscopy & imaging analysis
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