A BioID-derived proximity interactome for SARS-CoV-2 proteins

Danielle G. May; Laura Martin‐Sancho; Valesca Anschau; Sophie N. Liu; Rachel J. Chrisopulos; Kelsey L. Scott; Charles T. Halfmann; Ramon Díaz Peña; Dexter R. Pratt; Alexandre Rosa Campos; Kyle J. Roux

doi:10.3390/v14030611

Molecular BiologyVirusesMar 15, 2022

Peer-reviewed journal article

A BioID-derived proximity interactome for SARS-CoV-2 proteins

Danielle G. May iD, Laura Martin‐Sancho iD, Valesca Anschau iD, Sophie N. Liu iD, Rachel J. Chrisopulos, Kelsey L. Scott iD, Charles T. Halfmann iD, Ramon Díaz Peña, Dexter R. Pratt iD, Alexandre Rosa Campos iD, Kyle J. Roux iD

Abstract

The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here we use BioID to map the SARS-CoV-2 virus-host interactome using human lung cancer derived A549 cells expressing individual SARS-CoV-2 viral proteins. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are in association with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Collectively, these studies provide a valuable resource to potentially uncover novel SARS-CoV-2 biology and inform development of antivirals.

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