The protective effect of Capheic acid phenyl ester on hepatic ischemia-reperfusion injury in cholestatic rats

PURPOSECaffeic acid phenyl ester (CAPE), which is an active component of propolis, has antioxidant, antiproliferative, immunomodulatory and anti-inflammatory properties and has been used for many years. The protective effect against ischemic reperfusion injury in the cholestatic liver is unknown and it is aimed to determine its effect in this study.\n\nMATERIALS AND METHODSThree groups of 18 Wistar albino rats were divided into cholestasis, control and study groups with bile duct attachment. Intraperitoneal administration of caffeic acid phenyl ester at the dose of 30 mg/kg/ day started 15 days ago and continued until the second operation in the study group. On the seventh day, hepatic ischemia was performed for 30 minutes followed by reperfusion for 60 minutes. Serum, plasma and liver samples were taken. Laboratory analysis, tissue glutathione, malondialdehyde, and myeloperoxidase levels were evaluated.\n\nRESULTSSignificant reduction in the level of liver glutathione and a marked increase in malondialdehyde level and myeloperoxidase activity were observed in the cholestatic I / R group. After treatment, all parameters except serum bilirubin levels were reversed.\n\nCONCLUSIONSIntraperitoneal administration of CAPE may improve liver function and may reduce inflammation and oxidative stress in cholestatic I / R injury.