SCON - A Short Conditional intrON for conditional knockout with one-step zygote injection
Szu-Hsien Sam WuiD, Heetak LeeiD, Réka Szép-Bakonyi, Gabriele ColozzaiD, Ayse Boese, Krista R. GertiD, Natalia Hallay, Ji‐Hyun LeeiD, Jihoon KimiD, Yi Zhun ZhuiD, Margot M. L. LinsseniD, Sandra Pilat-Carotta, Peter HohensteiniD, Hans‐Christian TheussliD, Andrea PauliiD, Bon‐Kyoung KooiD
Abstract
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables rapid generation of conditional alleles via one-step zygote injection. SCON has conditional intronic function in various vertebrate species and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
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