Andrea Pauli

This study identifies SPARK as a conserved multi-protein complex essential for vertebrate fertilization, linking gamete recognition to membrane fusion.

The paper tests cross-species sperm-egg compatibility in fish by swapping Spaca6 and Izumo1 proteins between medaka and zebrafish, showing that specific molecular interactions are required for fertilization.

The paper identifies PITHD1 as an endogenous inhibitor of the 26S proteasome in dormant cells, revealing a triple-lock mechanism that prevents protein degradation during cellular dormancy.

The paper identifies a conserved trimeric complex of Izumo1, Spaca6, and Tmem81 essential for sperm-egg interaction in vertebrates, showing their role in male fertility and binding to the egg protein Bouncer.

The paper identifies a dual ribosomal system in zebrafish, showing maternal and somatic ribosomes are both active and can form hybrid ribosomes in the embryo.

The paper develops tRAM-seq to study tRNA expression and modification during zebrafish embryo development, revealing a reprogramming of the tRNA pool around gastrulation.

The paper profiles mitochondrial activity, morphology, and associated molecular changes during zebrafish embryogenesis, identifying increased ER-mitochondria contacts and mitochondrial fusion as key contributors to mitochondrial activation.

This study identifies eIF4E1b as a key factor in maternal mRNA dormancy by maintaining short polyA tails and repressing translation until needed in zebrafish embryos.

The paper compares DNA methylation dynamics in medaka and zebrafish embryos, showing conserved remodelling patterns without global erasure, and identifies conserved non-canonical methylation reprogramming.

The paper identifies specific amino acid residues and N-glycosylation patterns in Bouncer proteins that mediate species-specific sperm-egg compatibility between medaka and zebrafish.

This study uses SLAMseq to analyze maternal-to-zygotic transition in zebrafish, revealing early zygotic transcription and dynamics of maternal-zygotic gene expression.

This paper introduces SCON, a short conditional intron for conditional knockout via one-step zygote injection, showing its function across vertebrate species.

The paper identifies a self-generated Toddler gradient guided by Aplnr that directs mesodermal cell migration during zebrafish gastrulation.

This study shows that DCST1 and DCST2 are essential for sperm-egg fusion in mice and zebrafish, with mutations leading to male infertility.

This paper presents an improved RT-LAMP assay for SARS-CoV-2 detection that is rapid, sensitive, and suitable for both laboratory and home testing.

This study shows that zebrafish Spaca6 is essential for fertilization by regulating Dcst2 levels and enabling sperm-egg membrane binding.

This study uses reciprocal zebrafish-medaka hybrids to show that maternal factors primarily control the timing of zygotic genome activation.

The paper identifies maternally-deposited antisense piRNAs that silence transposons in zebrafish embryos by targeting young LTR retrotransposons and promoting H3K9me2/3 modification.

This study identifies SPACA4 as an essential sperm protein required for zona pellucida penetration during mammalian fertilization in mice.

This study identifies Ski7 as a post-transcriptional regulator that maintains low transcript levels of specific genes during the zebrafish oocyte-to-embryo transition, impacting fertility and redox processes.

This study shows PCF11 is critical for vertebrate development by promoting transcription termination and preventing transcriptional interference.

This study identifies Bouncer, a Ly6/uPAR protein in zebrafish oocytes, as essential for species-specific fertilization and shows it can enable cross-species fertilization between zebrafish and medaka.