Metabolomics of a Cysteinyl Leukotriene Receptor 1 (CysLTR1) Knockout Mouse Model by Analysis of Bronchoalveolar Lavage Fluid Using Gas Chromatography-time of Flight Mass Spectrometry.

Abstract

Cysteinyl leukotriene receptor 1 (CysLTR1), a potent lipid mediator, is known for its critical role in regulating inflammatory responses, particularly in asthma and airway diseases. While its function in immune cell recruitment has been previously reported, its broader impact on pulmonary metabolism remains largely unexplored. In this study, we investigated the metabolic consequences of CysLTR1 deletion in mice using GC-TOFMS-based metabolomics analysis of bronchoalveolar lavage fluid (BALF) from both CysLTR1 knockout (KO) and wild-type (WT) mice. The BALF from CysLTR1 KO mice exhibited significantly reduced levels of glucose, gluconic acid, sedoheptulose, D-xylose, glucosamine, glyceric acid, and 1-methylinosine, indicating impaired glucose uptake and dysregulation of glycolysis and gluconeogenesis. Further disruption of glucose-associated pathways, including the pentose phosphate pathway and purine metabolism, alongside reduced 1-methylinosine levels, suggests altered RNA turnover. In addition, decreases in butanoic acid, decan-2-ol, and 1-hexadecanol point to dysregulated fatty acid metabolism, potentially as a compensatory response to glucose deficiency. Altered levels of mandelic acid, glutaric acid, tricarballylic acid, and decan-2-ol, some of which are derived from corn-based diets also indicate changes in the pulmonary microbiome. Overall, the deletion of CysLTR1 significantly disrupts pulmonary metabolic homeostasis, affecting the metabolism of carbohydrates, lipids, amino acids, nucleotides, and microbial-derived metabolites.