Author

Joshua J. Hughes

University of ManchesterORCID 0000-0003-2335-5181Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:cell type annotation· 2 Assay:bulk RNA-seq· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:differential expression· 1 Assay:flow cytometry· 1 Organism:human· 1 Organism:mouse· 1

Frequent Co-Authors

Andrew S. MacDonaldiD· 3 shared Amanda J. L. RidleyiD· 2 shared Stefano A. P. ColomboiD· 2 shared Alexander Phythian‐AdamsiD· 1 shared Angela SimpsoniD· 1 shared Annabel J. CurleiD· 1 shared Benjamin Harrop· 1 shared Douglas P. DyeriD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 28 mutual co-author links

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3 shared papersImmunology

Andrew S. MacDonaldORCID

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3 papers

Recent·Most cited

Cell BiologyiSciencePeer-reviewedSep 21, 2026

Macrophages promote collagen deposition through circadian regulation of fibroblasts

Katherine Lowles, Xin-Yang Zhang, Xuefeng Yao…·Joan Chang

This study shows macrophages enhance collagen deposition in fibroblasts through circadian regulation, with M2 macrophages using PDGFA to strengthen circadian rhythms and increase collagen production.

DOI 10.1016/j.isci.2026.117573

proteomicsexperimental assay

𝕏 PostBluesky

ImmunologybioRxivPreprintJul 2, 2025

Pulmonary macrophage subsets display distinct metabolic responses to polarising stimuli in vivo

Joshua J. Hughes, Stefano A. P. Colombo, Lee M. Booty…·Andrew S. MacDonald

This study investigates how pulmonary macrophage subsets respond metabolically to polarizing stimuli in vivo, revealing distinct metabolic profiles between alveolar and interstitial macrophages.

DOI 10.1101/2025.07.02.662727

mouseflow cytometry

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ImmunologybioRxivPreprintFeb 26, 2025

Hallmarks and metabolic regulation of type 2 activated human lung macrophages

Amanda J. L. Ridley, Annabel J. Curle, Stefano A. P. Colombo…·Andrew S. MacDonald

This study identifies hallmark gene expression and metabolic profiles of type 2 activated human lung macrophages using primary tissue-derived cells.

DOI 10.1101/2025.02.26.640352

humanbulk RNA-seq

𝕏 PostBluesky