bioRxivDevelopmental BiologyPreprint
Verified the effectiveness of AsCpf1 system in a variety of vertebrate species
Zhuo LiiD, Zhaoying ShiiD, Nana FaniD, Yongqiang CheniD, Jing GuoiD, Jingchun WuiD, Hong SongiD, Shilong Chu, Kunlun Mo, Bentian Zhao, Zhen Ouyang, Dandan Tian, Shaoyang Zhao, Jieying ZhuiD, Jiekai CheniD, Yonglong CheniD, Liangxue LaiiD, Duanqing PeiiD
- Citations
- 4
Abstract
CRISPR/Cpf1 system is a novel genomic editing tool. Because of its more sophisticated components, and lower off-target rate, it has the potential to become a better gene-editing tool. Previous reports showed that CRISPR/Cpf1 could work effectively in multiple species. But our data show that AsCpf1 activity has a big difference in different vertebrates. Using in vitro experiments, we finally learned that the difference between species is due to temperature.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
Miguel A. Moreno-Mateos, Juan Pablo Fernández, … Antonio J. Giráldez·Nature CommunicationsPeer-reviewed·Dec 4, 2017
- Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array
Bernd Zetsche, Matthias Heidenreich, … Feng Zhang·Nature BiotechnologyPeer-reviewed·Feb 1, 2017
- A Survey of Genome Editing Activity for 16 Cpf1 orthologs
Bernd Zetsche, Jonathan G. Strecker, … Feng Zhang·bioRxiv·May 4, 2017
- Phosphorothioate-modified DNA oligonucleotides inactivate CRISPR-Cpf1 mediated genome editing
Bin Li, Chunxi Zeng, … Yizhou Dong·bioRxiv·Feb 6, 2018
- Comparison of DNA targeting CRISPR editors in human cells
Hongxin Huang, Weiqi Lv, … Ying Lin·bioRxiv·Oct 20, 2022
- Orthogonal CRISPR-Cas tools for genome editing, inhibition, and CRISPR recording in zebrafish embryos.
Paige Takasugi, Shengzhou Wang, … James A. Gagnon·GeneticsPeer-reviewed·Nov 4, 2021