Evaluation of anti-noroviral compounds on human norovirus replication using 2D monolayers of human intestinal organoids

Abstract

We evaluated the anti-noroviral effects of compounds including molnupiravir, on human norovirus (HuNoV) replication using 2D monolayers of human intestinal organoids (HIOs), which have been widely used in HuNoV infection studies. We found that these compounds exhibited lower antiviral activity in 2D HIO monolayers compared with that in apical-out 3D HIOs, a recently developed culture system for HuNoV. This suggests that the anti-noroviral efficacy of these compounds may vary depending on the assay system used.