M. K. Estes

Bile acid-sensitive human norovirus strains are susceptible to sphingosine-1-phosphate receptor 2 inhibition

This study shows that BA-dependent human norovirus strains require S1PR2 for infection, while BA-independent strains do not, suggesting S1PR2 as a potential therapeutic target.

RNA-dependent RNA polymerase of predominant human norovirus forms liquid-liquid phase condensates as viral replication factories

Functional Genomics of Gastrointestinal Escherichia coli Isolated from Patients with Cancer and Diarrhea

The paper investigates E. coli strains from cancer patients with diarrhea, finding novel adherence patterns and high antibiotic resistance gene carriage.

Intra- and Inter-host Evolution of Human Norovirus in Healthy Adults

The paper studies intra- and inter-host evolution of human norovirus in healthy adults using a capture-based sequencing approach, finding limited conserved immune pressure-driven evolution.

A Standardized Antiviral Pipeline for Human Norovirus in Human Intestinal Enteroids Demonstrates No Antiviral Activity of Nitazoxanide

This study tests nitazoxanide's antiviral activity against human noroviruses using a standardized pipeline in human intestinal enteroids, finding no significant antiviral effect.

Infant and Adult Human Intestinal Enteroids are Morphologically and Functionally Distinct

This study compares infant and adult human intestinal enteroids, finding significant transcriptomic, morphological, and functional differences that highlight their distinct characteristics.

Hepatobiliary organoids derived from leporids support the replication of hepatotropic lagoviruses

This study demonstrates that hepatobiliary organoids from leporids support replication of hepatotropic lagoviruses, with species-specific tropism observed in different host organoids.

Evaluation of heat inactivation of human norovirus in freshwater clams using human intestinal enteroids

This study evaluates heat inactivation of human norovirus in freshwater clams using human intestinal enteroids, finding that 1 minute at 90°C inactivates the virus.

Yersinia pseudotuberculosis YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers.

This study uses human ileal enteroid-derived monolayers to show that Yersinia pseudotuberculosis YopE prevents uptake by M cells and causes their extrusion, facilitating bacterial transcytosis.

The human nose organoid respiratory virus model: an ex-vivo human challenge model to study RSV and SARS-CoV-2 pathogenesis and evaluate therapeutics

This paper develops a non-invasive human nose organoid model to study RSV and SARS-CoV-2 infection and test therapeutics like palivizumab.

Dasabuvir inhibits human norovirus infection in human intestinal enteroids

This study identifies dasabuvir as an anti-HuNoV inhibitor using human intestinal enteroids, showing activity against HuNoV, SARS-CoV-2, and RVA.

New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids

This paper optimizes human intestinal enteroid culture conditions to enhance cultivation of diverse human norovirus strains and improves replication consistency over time.

Coronin-1 is necessary for enteric pathogen-induced transcytosis across human ileal enteroid monolayers expressing M cells

This study shows that coronin 1a is required for M cell uptake and transcytosis of pathogens in human ileal enteroids, but not commensal bacteria.

Isolation, culture and maintenance of rabbit intestinal organoids, and organoid-derived cell monolayers

This paper establishes a protocol for isolating and maintaining rabbit intestinal organoids and monolayers, demonstrating their ability to mimic intestinal epithelium and support pathogen research.

Obesity phenotypes are preserved in intestinal stem cell enteroids from morbidly obese patients

The paper identifies obesity-related phenotypes in intestinal stem cell enteroids from morbidly obese patients and shows they retain patient-specific traits post-bariatric surgery.

Rotavirus Induces Intercellular Calcium Waves Through ADP Signaling

This study shows rotavirus induces intercellular calcium waves via ADP signaling, which amplifies diarrheal symptoms and can be blocked by P2Y1 antagonists or knockout.

Human Rotavirus Diarrhea Is Associated with Altered Trafficking and Expression of Apical Membrane Transport Proteins

Rotavirus Calcium Dysregulation Manifests as Dynamic Calcium Signaling in the Cytoplasm and Endoplasmic Reticulum

This study characterizes dynamic calcium signaling during rotavirus infection, showing increased cytosolic calcium spikes and ER release, with NSP4 and SOCE playing key roles.

Human intestinal enteroids with inducible neurogenin-3 expression as a novel model of gut hormone secretion

This paper develops a method to increase enteroendocrine cells in human intestinal enteroids using inducible neurogenin-3 expression, enabling functional studies of gut hormone secretion.

A novel human enteroid-anaerobe co-culture system to study microbial-host interaction under physiological hypoxia

This paper introduces a co-culture system to study host-microbe interactions under physiological hypoxia using human intestinal enteroids and anaerobic bacteria.

Epithelial WNT2B and Desert Hedgehog are necessary for human colonoid regeneration after bacterial cytotoxin injury

Bile acids target proteolipid nano-assemblies of EGFR and phosphatidic acid in the plasma membrane for stimulation of MAPK signaling

This study shows bile acids like DCA alter lipid nano-assemblies in the plasma membrane, promoting EGFR clustering and MAPK signaling in Caco-2 cells and human intestinal enteroids.