This study identifies type I interferon signaling as inducing a delayed antiproliferative response in Calu-3 cells during SARS-CoV-2 infection, which may impair alveolar regeneration.

bioRxiv · Feb 28, 2023 · PreprintMicrobiology

Amino acid substitutions in norovirus VP1 dictate cell tropism via an attachment process dependent on membrane mobility.

J. T. Mills, S. C. Minogue, J. S. Snowden, K. E. Carnahan, D. J. Rowlands, N. J. Stonehouse, C. E. Wobus, M. R. Herod

This study identifies an amino acid substitution in norovirus VP1 that enhances cellular attachment and alters tropism by affecting receptor recruitment and membrane mobility.

bioRxiv · Feb 17, 2023 · PreprintMicrobiology

Neutrophils prime unique transcriptional responses in intestinal organoids during infection with nontyphoidal Salmonella enterica serovars

A.-L. E. Lawrence, R. P. Berger, D. R. Hill, S. Huang, V. K. Yadagiri, B. Bons, C. Fields, J. S. Knight, C. E. Wobus, J. R. Spence, V. B. Young, B. H. Abuaita, M. X. O'Riordan

This study investigates how human neutrophils modulate host responses to two nontyphoidal Salmonella serovars using intestinal organoids and transcriptomics.

bioRxiv · Aug 9, 2022 · PreprintImmunology

In Vitro Evaluation and Mitigation of Niclosamide's Liabilities as a COVID-19 Treatment.

J. W. Wotring, S. M. McCarty, K. Shafiq, C. J. Zhang, T. Nguyen, S. R. Meyer, R. Fursmidt, C. Mirabelli, M. C. Clasby, C. E. Wobus, M. J. O'Meara, J. Z. Sexton

This study evaluates niclosamide's limitations as a COVID-19 treatment and identifies analogs with improved potency and reduced toxicity.

bioRxiv · Jun 24, 2022Microbiology

ARF6 is a host factor for SARS-CoV-2 infection in vitro

C. Mirabelli, E. J. Sherman, J. Bragazzi Cunha, J. W. Wotring, J. El Saghir, J. Harder, J. Z. Sexton, B. T. Emmer, C. E. Wobus

This study identifies ARF6 as a host factor critical for SARS-CoV-2 entry and infection in vitro, showing its importance across different cell models.

bioRxiv · Jun 9, 2022 · PreprintMicrobiology

Human norovirus efficiently replicates in differentiated 3D-human intestinal enteroids

C. Mirabelli, N. Santos-Ferreira, M. G. Gillilland, R. J. Cieza, J. Colacino, J. Z. Sexton, S. Taube, J. Rocha-Pereira, C. E. Wobus

This paper develops a simplified protocol for infecting 3D-human intestinal enteroids with human norovirus, showing improved infection efficiency and host response analysis.

bioRxiv · Jun 9, 2022Microbiology

Human neutrophils direct epithelial cell extrusion to enhance intestinal epithelial host defense during Salmonella infection

A.-L. E. Lawrence, R. P. Berger, D. R. Hill, S. Huang, V. K. Yadagiri, B. Bons, C. Fields, G. J. Sule, J. S. Knight, C. E. Wobus, J. Spence, V. Young, M. X. O'Riordan, B. Abuaita

This study shows human neutrophils enhance intestinal epithelial cell extrusion to reduce Salmonella burden during infection, beyond direct antimicrobial activity.

bioRxiv · Mar 25, 2022Microbiology

Morphological Cell Profiling of SARS-CoV-2 Infection Identifies Drug Repurposing Candidates for COVID-19

C. Mirabelli, J. W. Wotring, C. J. Zhang, S. M. McCarty, R. Fursmidt, T. Frum, N. S. Kadambi, A. T. Amin, T. R. O'Meara, C. D. Pretto-Kernahan, J. R. Spence, J. Huang, K. D. Alysandratos, D. N. Kotton, S. K. Handelman, , J. Z. Sexton

This study identifies 17 FDA-approved compounds with antiviral efficacy against SARS-CoV-2 using high-throughput screening and finds lactoferrin as a promising drug candidate with multiple antiviral mechanisms.

Proceedings of the National Academy of Sciences · Aug 19, 2021Peer-reviewedCell Biology

Human norovirus infection of primary B cells triggers immune activation in vitro

C. Mirabelli, M. K. Jones, V. Young, A. Kolawole, I. Owusu, M. Shan, B. H. Abuaita, I. Grigorova, S. K. Lundy, C. A. Lyssiotis, V. K. Ward, S. M. Karst, C. E. Wobus

This study shows human norovirus infects primary B cells, induces functional changes, and the NS1 protein affects B cell proliferation and metabolism in vitro.

bioRxiv · May 14, 2021 · PreprintMicrobiology

Comparative analysis of public RNA-sequencing data from human intestinal enteroid (HIEs) infected with enteric RNA viruses identifies universal and virus-specific epithelial responses

R. J. Cieza, J. L. Golob, J. A. Colacino, C. E. Wobus

The paper analyzes public RNA-seq data from HIEs infected with enteric viruses to identify common and virus-specific epithelial responses.

bioRxiv · Mar 30, 2021Microbiology

Host-virus chimeric events in SARS-CoV2 infected cells are infrequent and artifactual

B. Yan, S. Chakravorty, C. Mirabelli, L. Wang, J. L. Trujillo-Ochoa, D. Chauss, D. Kumar, M. S. Lionakis, M. R. Olson, C. E. Wobus, B. Afzali, M. Kazemian

This study investigates host-virus chimeric (HVC) events in SARS-CoV2 RNA-seq data and finds they are rare and likely artifacts, not evidence of viral integration.

bioRxiv · Feb 17, 2021Microbiology

Natural IgA and TNFRSF13B polymorphism: a double edged sword fueling balancing selection

J. L. Platt, M. G. de Mattos Barbosa, D. Huynh, A. R. Leferts, J. Katta, C. Kharas, P. Freddolino, C. M. Bassis, C. E. Wobus, R. Geha, R. J. Bram, G. Nunez, N. Kamada, M. Cascalho

The paper investigates how a dominant-negative variant of TNFRSF13B confers resistance to enteric pathogens by reducing natural IgA, suggesting balancing selection due to its dual role in immunity and pathogenesis.

bioRxiv · Jan 29, 2021 · PreprintImmunology

Comparative transcriptional profiling of the early host response to infection by typhoidal and non-typhoidal Salmonella serovars in human intestinal organoids

B. H. Abuaita, A.-L. E. Lawrence, R. P. Berger, D. R. Hill, S. Huang, V. K. Yadagiri, B. Bons, C. Fields, C. E. Wobus, J. R. Spence, V. B. Young, M. O'Riordan

This study compares host transcriptional responses to typhoidal and non-typhoidal Salmonella serovars in human intestinal organoids, revealing distinct gene expression patterns and immune responses.

bioRxiv · Nov 25, 2020 · PreprintMicrobiology

Salmonella enterica serovar Typhimurium SPI-1 and SPI-2 shape the transcriptional landscape of epithelial cells in a human intestinal organoid model system

A.-L. E. Lawrence, B. H. Abuaita, R. P. Berger, D. R. Hill, S. Huang, V. K. Yadagiri, B. Bons, C. Fields, C. E. Wobus, J. R. Spence, V. B. Young, M. O'Riordan

This study uses human intestinal organoids to investigate how Salmonella SPI-1 and SPI-2 T3SSs influence host transcriptional responses during infection, revealing distinct and overlapping roles in inflammation and cell cycle suppression.

bioRxiv · Oct 5, 2020Microbiology

Macrophage Inflammatory State Influences Susceptibility to Lysosomal Damage

A. O. Wong, M. Marthi, I. Owusu, C. E. Wobus, J. Swanson

This study shows that macrophage susceptibility to lysosomal damage varies with their inflammatory state and depends on the type I IFN response.

bioRxiv · May 29, 2020Immunology

A Novel Small Animal Model of Norovirus Diarrhea

A. N. Roth, E. W. Helm, C. Mirabelli, E. Kirsche, J. Smith, L. B. Eurell, S. Ghosh, N. Altan-Bonnet, C. E. Wobus, S. M. Karst

This study identifies a murine norovirus that causes self-resolving diarrhea in neonatal mice without systemic disease, mirroring human norovirus infection and establishing a new animal model.

bioRxiv · Mar 19, 2020 · PreprintMicrobiology

Central carbon metabolism is an intrinsic factor for optimal replication of a norovirus

K. Passalacqua, J. Lu, I. G. Goodfellow, A. O. Kolawole, J. R. Arche, R. J. Maddox, M. O'Riordan, C. E. Wobus

This study shows that central carbon metabolism is essential for optimal murine norovirus replication and suggests metabolism as a therapeutic target for norovirus infections.

bioRxiv · Oct 3, 2018 · PreprintMicrobiology