Mary Kolb Estes

Development of fluorescent and affinity tagged variants of rotavirus nonstructural protein 4 using the reverse genetics system

This paper develops fluorescent and affinity-tagged rotavirus NSP4 variants using reverse genetics to study its function during infection.

Mutational Analysis of Human Norovirus VP2 Elucidates Critical Molecular Interactions for Virus Assembly

This paper identifies critical molecular interactions of VP2 in HuNoV assembly through mutational analysis and computational modeling.

Functional Diversity in GII.4 Norovirus Entry: HBGA Binding and Capsid Clustering Dynamics

The paper compares GII.4 norovirus variants to determine if clustering and entry mechanisms are conserved, finding clustering enhances entry and is mediated by specific residues and lipid rafts.

Overcoming host restrictions to enable continuous passaging of human noroviruses in human intestinal enteroids

This study identifies host restriction factors limiting HuNoV passaging in HIEs and demonstrates that TAK-779 enhances viral replication and enables continuous passaging.

The Orai channel inhibitor BTP2 restricts Tulane virus and human norovirus replication independent of store-operated calcium entry

This study shows that BTP2 inhibits Tulane virus and human norovirus replication by targeting structural proteins, not through SOCE, and establishes a system for antiviral screening.

Conformational flexibility is a critical factor in designing broad-spectrum human norovirus protease inhibitors

This study identifies conformational flexibility in GII proteases as a key factor affecting inhibitor potency, guiding the design of broad-spectrum norovirus protease inhibitors.

2'-Fucosyllactose Inhibits Human Norovirus Replication in Human Intestinal Enteroids

This paper shows that 2'-fucosyllactose inhibits replication of a dominant human norovirus strain in intestinal enteroids from adult and pediatric donors.

Insights into Human Norovirus Cultivation in Human Intestinal Enteroids

This paper reports the cultivation of human noroviruses in human intestinal enteroids, revealing segment- and strain-specific differences in viral replication and highlighting the utility of genetically modified enteroids for broader strain cultivation.

Complete Genomic Characterization of Global Pathogens, Respiratory Syncytial Virus (RSV), and Human Norovirus (HuNoV) Using Probe-based Capture Enrichment.

This paper presents a probe-based capture enrichment method to obtain full-length genomes of RSV and HuNoV from clinical samples, achieving high completeness and improving variant tracking.

Drivers of Transcriptional Variance in Human Intestinal Epithelial Organoids

This study identifies major sources of transcriptomic variation in human intestinal organoids, showing that culture methods like substrate and format have large effects compared to experimental manipulations.