An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

M. Ryan Corces; Alexandro E. Trevino; Emily G. Hamilton; Peyton Greenside; Nasa Sinnott-Armstrong; Sam S. Vesuna; Ansuman T. Satpathy; Adam J. Rubin; Kathleen S. Montine; Beijing Wu; Arwa S. Kathiria; Seung Woo Cho; Maxwell R. Mumbach; Ava C. Carter; Maya M. Kasowski; Lisa Ann Orloff; Viviana I. Risca; Anshul Bharat Kundaje; Paul A. Khavari; Thomas Jude Montine; William James Greenleaf; Howard Y. Chang

doi:10.1038/nmeth.4396

GenomicsNature MethodsAug 28, 2017

Peer-reviewed journal article

An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

M. Ryan Corces iD, Alexandro E. Trevino iD, Emily G. Hamilton iD, Peyton Greenside, Nasa Sinnott-Armstrong iD, Sam S. Vesuna iD, Ansuman T. Satpathy iD, Adam J. Rubin iD, Kathleen S. Montine iD, Beijing Wu, Arwa S. Kathiria, Seung Woo Cho iD, Maxwell R. Mumbach iD, Ava C. Carter iD, Maya M. Kasowski iD, Lisa Ann Orloff iD, Viviana I. Risca iD, Anshul Bharat Kundaje iD, Paul A. Khavari iD, Thomas Jude Montine iD, William James Greenleaf iD, Howard Y. Chang iD

Abstract

We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol enables chromatin accessibility profiling from archival frozen tissue samples and 50 m sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.

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