Hao Zhang

Single-crypt evolutionary trajectories of human sporadic and hereditary colorectal precancers

This study compares the genomic evolution of sporadic and hereditary colorectal precancers using single-crypt whole-genome sequencing, revealing distinct mutation patterns and evolutionary trajectories.

Polyclonal origins of human premalignant colorectal lesions

This study shows that many premalignant colorectal polyps contain multiple genetic lineages, indicating polyclonal origins rather than single-cell expansion.

Clonal expansion dictates the efficacy of mitochondrial lineage tracing in single cells

The paper investigates the reliability of mitochondrial DNA variants as lineage markers, finding that most are pre-existing heteroplasmy rather than de novo mutations, and introduces a score to identify reliable markers.

txci-ATAC-seq, a massive-scale single-cell technique to profile chromatin accessibility

The paper introduces txci-ATAC-seq, a high-throughput single-cell ATAC-seq method, and demonstrates its application across multiple tissues and genetic backgrounds.