Major functional bias for mitochondrial complexes in genome-wide CRISPR screens

Mahfuzur Rahman; Maximilian Billmann; Michael Costanzo; Michael Aregger; Amy H.Y. Tong; Katherine Chan; Henry N. Ward; Kevin R. Brown; Brenda Andrews; Charles M. Boone; Jason Moffat; Chad L. Myers

doi:10.15252/msb.202010013

Systems BiologyMolecular Systems BiologyMay 1, 2021

Peer-reviewed journal article

Major functional bias for mitochondrial complexes in genome-wide CRISPR screens

Mahfuzur Rahman iD, Maximilian Billmann iD, Michael Costanzo iD, Michael Aregger iD, Amy H.Y. Tong iD, Katherine Chan iD, Henry N. Ward iD, Kevin R. Brown iD, Brenda Andrews iD, Charles M. Boone iD, Jason Moffat iD, Chad L. Myers iD

PDF DOI Preprint

Post to X

Threads on X Post to Bluesky Threads on Bluesky

Abstract

We present FLEX (Functional evaluation of experimental perturbations), a pipeline that leverages several functional annotation resources to establish reference standards for benchmarking human genome-wide CRISPR screen data and methods for analyzing them. We apply FLEX to analyze data from the diverse cell line screens generated by the DepMap project. We identify a dominant mitochondria-associated signal, which our time-resolved CRISPR screens and analysis suggests may reflect screen dynamics and protein stability effects rather than genetic dependencies.