Single-cell mass spectrometry imaging combined with immunofluorescence reveals neutrophil heterogeneity in inflammation

Abstract

Multimodal single-cell approaches allow for a holistic analysis of complex biological systems. In this study, we developed a novel single-cell analysis pipeline integrating immunofluorescence-based protein with mass spectrometry imaging-based lipid analysis of circulating human neutrophils. The combination of both modalities identified the emergence of pathogenic neutrophils in liver cirrhosis patients thus epitomizing the potential of this technology to reveal cellular phenotypes in health and disease.