Rupsa Datta

Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy

This paper introduces a photon pooling technique for faster single-cell FLIM analysis without machine learning, achieving single-cell metabolic data every second and 15x faster mosaic acquisition.

FLIM Playground: An interactive, end-to-end graphical user interface for analyzing single-cell fluorescence lifetime data

FLIM Playground provides an interactive, open-source platform for analyzing single-cell fluorescence lifetime data, offering unified workflows and real-time analysis tools validated on cancer cell line datasets.

Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems

This study uses optical metabolic imaging to show neutrophils rapidly shift to a reduced redox state upon activation, with heterogeneous responses across cells and donors.

Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response

This study shows that mature neutrophils can rapidly reactivate mitochondrial metabolism in response to stimuli, impacting their function and suggesting therapeutic potential for mitochondrial pyruvate carrier inhibitors.

GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18

This study shows that GATA1-deficient iPSC-derived neutrophils have enhanced antifungal activity against Aspergillus fumigatus, mediated by CD18 integrin.