bioRxivBiochemistryPreprint
Identification of Inhibitors of Chikungunya virus nsP2 ATPase
Hernán A. Navarro, John E. Scott, Ginger R. SmithiD, Pegah Ghiabi, Elisa GibsoniD, P. LoppnauiD, Rachel HardingiD, Mohammad Anwar HossainiD, M. BoseiD, Kenneth H. PearceiD, Eric M. MerteniD, Timothy Mark WillsoniD, Peter J. BrowniD
Abstract
Non-structural protein 2 (nsP2), which plays an essential role in replication of CHIKV, contains a protease, helicase, and methyltransferase-like domain. We executed a simple a screen using malachite green to detect compounds that decreased ATP hydrolysis and tested a library of diverse compounds to find inhibitors of CHIKV nsP2 helicase.
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