Evaluation of N6-adenine DNA-immunoprecipitation-based genomic profiling in eukaryotes
Brian M. Debo, Benjamin J. MalloryiD, Andrew B. StergachisiD
Abstract
The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, using a validated highly selective anti-DNA-m6A antibody we expose distinct patterns of DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.
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