bioRxivImmunology
One-step generation of monoclonal B cell receptor mice capable of class switch recombination and somatic hypermutation
J. T. Jacobsen, L. Mesin, S. Markoulaki, C. B. Cavazzoni, D. Bousbaine, R. Jaenisch, G. D. Victora
Abstract
We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing pre-rearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR/Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR and affinity maturation.
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