Nature CommunicationsCell BiologyPeer-reviewed journal article
Outcome-Driven Microscopy: Closed-Loop Optogenetic Control of Cell Biology
Josiah B. PassmoreiD, Alfredo RatesiD, Jakob SchröderiD, Menno T. P. van LaarhoveniD, Vincent J. W. Hellebrekers, Henrik G. van HoefiD, Antonius J. M. Geurts, Wendy van Straaten, Wilco NijenhuisiD, Florian BergeriD, Carlas SmithiD, Ihor SmaliD, Lukas C. KapiteiniD
Abstract
Smart microscopy is transforming biological imaging by integrating real-time analysis with adaptive acquisition to enhance imaging efficiency. Whereas many emerging implementations are event-driven and focus on on-demand data acquisition to reduce phototoxicity, we here present outcome-driven microscopy, which combines smart microscopy with optogenetics to achieve subcellular spatiotemporal control of biology to predefined outcomes. We validate this approach using light-based control of cell migration and nucleocytoplasmic transport, and demonstrate unprecedented spatiotemporal control over cellular behaviour.
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