Lukas C. Kapitein

This study uses volumetric bioprinting to enable post-printing optogenetic stimulation of cells within hydrogel constructs, demonstrating spatial control over light-responsive proteins and gene expression.

The paper introduces nuclear PIMs to study nuclear quality control and shows that proteasome and VCP disaggregate nuclear aggregates, with subunits exported to the cytosol for aggrephagy.

This study uses expansion microscopy to analyze three-dimensional ultrastructure of intestinal tissues and organoids, revealing disease-specific phenotypes in a microvillus inclusion disease model.

This paper benchmarks predictive modeling and control techniques with optogenetics to steer nucleocytoplasmic transport in mammalian cells, showing robust performance against cell-to-cell variation.

This study investigates the roles of CSTN1 and CSTN3 in axonal cargo transport, finding they coordinate TGN exit and regulate RAB6A levels to influence Golgi organization and trafficking.

This paper proposes a conceptual framework and biophysical model to explain how microtubule orientation emerges in developing neurons, showing how geometrical effects, parallel amplification, and polarization contribute to axon-dendrite differentiation.

This paper introduces a miRNA feedback circuit to control synthetic receptor activity, showing tunable and autonomous regulation of synNotch receptor expression.

The paper uses expansion microscopy to map the 3D microtubule organization in human airway multiciliated cells, revealing the role of NEDD1 in basal foot-dependent microtubule organization.

This study shows that fasting improves PLN R14del cardiomyopathy by restoring lysosomal function and reducing SR-derived vesicle accumulation.

This study develops a method to differentiate human nasal epithelial cells into airway-like tissues using small molecules, creating disease models for respiratory conditions.

This paper reviews current smart microscopy implementations and proposes a framework for interoperability to improve collaboration and standardization in the field.

This study shows taxanes induce conformational changes in microtubules, releasing GEF-H1 and activating RhoA signaling, leading to actin remodeling.

This paper introduces outcome-driven microscopy, combining optogenetics and smart microscopy to achieve precise control over cellular behavior through predefined outcomes.

This study uses expansion microscopy to show SARS-CoV-2 exits cells via Rab27-dependent amphisome fusion, identifying a potential therapeutic target.

The paper describes the immortalization of human nasal and bronchial airway epithelial cells using hTERT overexpression, enabling long-term genome editing studies.

This study investigates how stable microtubules reorient during neuronal development in rat hippocampal neurons, revealing a shift from plus-end-out to minus-end-out orientation.

This paper introduces SIMCODE, a method combining modulation patterns and deep learning to enhance single-molecule localization microscopy, achieving better resolution and performance in high-density samples.

This paper introduces a method to quantify intensity distribution in 3D and 2D microscopy data using spherical harmonics, demonstrating its application across various biological systems.

The paper shows Aurora B regulates abscission speed in stem cells by controlling microtubule stability through a feedback loop.

The paper shows that microtubule lattice spacing affects kinesin-1 binding, with expanded lattices preferentially bound by kinesin-1.

This study identifies axonal ER tubules and P180/RRBP1 as regulators of local mRNA translation in axons, showing their role in ribosome interactions and proteome dynamics.