bioRxivNeurosciencePreprint
A Human iPSC-derived Organoid System to Model Myelin Destruction and Repair
Shwathy RamesaniD, Joel Mason, Molly E. V. SwansoniD, Samuel A. Mills, Bradley J. TurneriD, Matt RutariD, David G. GonsalveziD, Samantha K. BartoniD
Abstract
We engineered a novel human iPSC-derived organoid system enriched with mature, myelinating oligodendrocytes and functionally reactive microglia. This model enables the study of human CNS remyelination following a demyelinating insult, encapsulating: myelin fragmentation, microglial clearance of myelin debris and oligodendrocyte genesis, differentiation, and axon ensheathment. This system provides a powerful and unparalleled capacity to interrogate complex human cell behaviour, relevant to those studying glial biology and neurological disease mechanisms.
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