Scientific ReportsGenomicsPeer-reviewed journal article
Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells.
Jan P. GerlachiD, Jessie A.G.L. van BuggenumiD, Sabine E.J. Tanis, Mark Hogeweg, Branco M. H. HeutsiD, Mauro J. MuraroiD, Lisa ElzeiD, Francesca RivelloiD, Agata RakszewskaiD, Alexander van OudenaardeniD, Wilhelm T. S. HuckiD, Hendrik G. StunnenbergiD, Klaas W. MulderiD
Abstract
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogenenous cellular responses to environmental signals at the mRNA and phospho-proteome level.
- organism: human
- assay: proteomics
- assay: bulk RNA-seq
- research focus: cell type annotation
- research focus: differential expression
- approach: experimental assay
- Citations
- 104
- FWCI
- 3.97
- Field percentile
- top 5%
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