Intranasal ChAdOx1 nCoV-19/AZD1222 vaccination reduces shedding of SARS-CoV-2 D614G in rhesus macaques
N. van Doremalen, J. Purushotham, J. Schulz, M. Holbrook, T. Bushmaker, A. Carmody, J. Port, K. C. Yinda, A. Okumura, G. Saturday, F. Amanat, F. Krammer, P. Hanley, B. Smith, J. Lovaglio, S. Anzick, K. Barbian, C. Martens, S. C. Gilbert, T. Lambe, V. Munster
Abstract
Intramuscular vaccination with ChAdOx1 nCoV-19/AZD1222 protected rhesus macaques against pneumonia but did not reduce shedding of SARS-CoV-2. Here we investigate whether intranasally administered ChAdOx1 nCoV-19 reduces shedding, using a SARS-CoV-2 virus with the D614G mutation in the spike protein. Viral load in swabs obtained from intranasally vaccinated hamsters was significantly decreased compared to controls and no viral RNA or infectious virus was found in lung tissue, both in a direct challenge and a transmission model. Intranasal vaccination of rhesus macaques resulted in reduced shedding and a reduction in viral load in bronchoalveolar lavage and lower respiratory tract tissue. In conclusion, intranasal vaccination reduced shedding in two different SARS-CoV-2 animal models, justifying further investigation as a potential vaccination route for COVID-19 vaccines.
02 /Specification & Classification
Click any tag to browse matching papers- Organism
- Assay
- Research Focus
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A single intranasal dose of chimpanzee adenovirus-vectored vaccine protects against SARS-CoV-2 infection in rhesus macaques
A. O. Hassan, F. Feldmann, … M. S. Diamond·bioRxiv·Jan 26, 2021
- ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
N. van Doremalen, T. Lambe, … V. Munster·bioRxiv·May 13, 2020
- Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2 in Preclinical Animal Models
J.-G. Park, F. S. Oladunni, … M. Munir·bioRxiv·Jan 8, 2021
- A single intranasal or intramuscular immunization with chimpanzee adenovirus vectored SARS-CoV-2 vaccine protects against pneumonia in hamsters.
T. Bricker, T. Darling, … A. Boon·bioRxiv·Dec 2, 2020
- A single intranasal dose of chimpanzee adenovirus-vectored vaccine confers sterilizing immunity against SARS-CoV-2 infection
A. O. Hassan, N. M. Kafai, … M. S. Diamond·bioRxiv·Jul 16, 2020
- Intradermal-delivered DNA vaccine provides anamnestic protection in a rhesus macaque SARS-CoV-2 challenge model
A. Patel, J. Walters, … K. E. Broderick·bioRxiv·Jul 28, 2020