bioRxivGenomicsPreprint
Gene editing of the multi-copy H2A.B gene family by a single pair of TALENS
Nur Diana AnuariD, Matthew A. FieldiD, Sebastian KurscheidiD, Lei ZhangiD, Edward J. RebariD, Philip D. GregoryiD, Josephine BowlesiD, Peter A. KoopmaniD, David John TremethickiD, Tatiana SobolevaiD
Abstract
In view of the controversy related to the generation of off-target mutations by gene editing approaches, we tested the specificity of TALENs by disrupting a multi-copy gene family using only one pair of TALENS. We show here that TALENS do display a high level of specificity by simultaneously knocking out the function of the three genes that encode for H2A.B.3. This represents the first described knockout of this histone variant.
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