Beau R. Webber

A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells

This paper develops a base editing platform to efficiently and safely engineer CAR T cells with multiple gene knockouts and insertions.

Modeling CISH-KO TIL therapy: T-cell persistence and endogenous competition determine response in gastrointestinal cancer

This study models CISH-KO TIL therapy in gastrointestinal cancer, finding that T-cell persistence and endogenous competition are key determinants of treatment response.

Efficient multiplex non-viral engineering and expansion of polyclonal γδ CAR-T cells for immunotherapy

This paper develops a scalable, non-viral method to engineer and expand polyclonal γδ CAR-T cells, showing their functional efficacy and subset-specific responses in vivo.

Mechanistic modeling predicts efficacy of CISH knockout in tumor-infiltrating lymphocytes with synergistic gene editing

The paper develops a mechanistic model to predict the efficacy of CISH knockout in TILs and identifies synergistic gene targets to enhance T cell activation.

Engineering physically optimized T cells for increased sampling of complex tumor microenvironments

This study physically optimizes T cells to enhance their ability to sample and engage with pancreatic ductal adenocarcinoma tumor cells, improving therapeutic outcomes.

Complement Factor H (Y402H) polymorphism for age-related macular degeneration alters retinal lipids

This study investigates how the CFH Y402H polymorphism and cigarette smoke extract affect lipid metabolism in iPSC-derived RPE cells, revealing genotype-dependent differences and CSE-induced lipid changes.

Bcl-2 protein Noxa is required for metabolic reprogramming to glutamine dependence and for apoptosis in stimulated human CD8+ T cells

This study identifies Noxa's role in metabolic reprogramming to glutamine dependence in CD8+ T cells, independent of its canonical apoptotic function, and suggests its potential as an immunotherapeutic target.

Installation of Dominant-Negative Mutations in FAS and TGFβR2 via Base Editing in Primary T Cells

This paper uses base editing to introduce dominant-negative mutations in FAS and TGFβR2 in primary T cells, enhancing their anti-tumor efficacy and persistence in solid tumor models.

Leukemia escapes immunity by imposing a Type-1 regulatory program on neoantigen-specific CD4+ T cells.

This paper shows leukemia induces neoantigen-specific CD4+ T cells to become regulatory (Tr1) cells, which suppress cytotoxic T cells and promote relapse, and repolarizing these cells to Th1 states eradicates residual disease.

Off-the-shelf allogeneic polyclonal CD38KO/CD38-CAR γδT cells for the treatment of T cell malignancies

This paper develops CD38KO/CD38-CAR γδT cells for T cell malignancies, showing they effectively target T-ALL without causing fratricide or GvHD.

Precision Enhancement of CAR-NK Cells through Non-Viral Engineering and Highly Multiplexed Base Editing

This study enhances CAR-NK cell functionality using non-viral base editing and transposon integration, showing improved therapeutic potential in Burkitt's lymphoma models.

CISH, a novel intracellular immune checkpoint, in comparison and combination to existing and emerging cancer immune checkpoints

This study shows that deleting CISH enhances T cell anti-cancer functions more than other intracellular checkpoints, improving tumor antigen recognition and killing in CAR-T cells.

Engineering Memory T Cells as a platform for Long-Term Enzyme Replacement Therapy in Lysosomal Storage Disorders

This study demonstrates that genetically engineered memory T cells can deliver enzyme replacement therapy for mucopolysaccharidosis type I, reducing GAG levels in multiple tissues for up to 22 weeks.

MultiEditR: An easy validation method for detecting and quantifying RNA editing from Sanger sequencing

MultiEditR is a tool that detects and quantifies RNA editing from Sanger sequencing, offering a cost-effective validation method for RNA editing sites.

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

This study develops a method to efficiently edit multiple genes in human T cells using base editors, reducing off-target effects and improving safety for immunotherapy.

GeneWeld: a method for efficient targeted integration directed by short homology

GeneWeld enables efficient and precise targeted integration in multiple species using CRISPR/Cas9 with minimal homology arms.

Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

This paper describes optimized CRISPR/Cas9 genome engineering of primary human B cells, achieving high-efficiency gene knockout and site-specific integration.

EditR: A novel base editing quantification software using Sanger sequencing

EditR quantifies base editing efficiency from Sanger sequencing data, offering accuracy and cost-effectiveness compared to existing methods.