Photosynthetic Processes and Mechanisms

Conserved and Lineage-Specific Roles of KEA-Mediated Ion Homeostasis in Chlamydomonas

Disruption of KEA-mediated chloroplast ion homeostasis in Chlamydomonas reinhardtii reveals conserved and lineage-specific control of plastid rRNA processing and cell cycle progression.

A genome-wide CRISPR screen reveals how diatoms thrive in dynamic light

A genome-wide CRISPR screen in diatoms identifies genes critical for survival under different light conditions, including a novel gene involved in cyclic electron flow.

RubiSpec-MS: Determination of Rubisco CO 2 /O 2 Specificity Factor using Liquid Chromatography-Mass Spectrometry

RubiSpec-MS measures Rubisco's SC/O using LC-MS/MS, offering a faster, more accessible alternative to radiolabeled methods with comparable accuracy.

A bifunctional coiled-coil protein generates the membrane-within-condensate architecture of the CO2-fixing pyrenoid

The paper identifies MITH1 as a protein that anchors thylakoid membranes into the pyrenoid condensate, revealing how membranes are integrated into biomolecular condensates.

Inositol Pyrophosphates Mediate Chloroplast Lipid Remodeling and Nuclear Gene Repression during High-Light Acclimation in Chlamydomonas reinhardtii

Loss of PSAE redirects PGRL1 to photosystem I and enhances PGR5-dependent cyclic electron transfer in Arabidopsis

This study shows that loss of PSAE in Arabidopsis enhances PGR5-dependent cyclic electron transfer and redirects PGRL1 to PSI, while NDH supports CO2 fixation under disrupted PSI conditions.

Exploring Thylakoid Emergence: Evolution of Membrane Biogenesis and Photosystem II assembly in early diverging Cyanobacteria

This study investigates the evolutionary origin of thylakoid membranes in cyanobacteria by analyzing phylogenomic data and structural models of membrane trafficking and Photosystem II assembly.

Deciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes

The paper introduces a crosslinking-MS strategy to map native protein interactions in functional thylakoid membranes from Arabidopsis and spinach while preserving photosynthetic activity.

Losing genes, gaining edits: how relaxed selection and inverted repeat expansion shape RNA editing in Schizaeaceae plastomes

This study examines RNA editing in Schizaeaceae plastomes, finding that relaxed selection and inverted repeat expansion contribute to increased C-to-U edits and altered gene expression.

PGR5 is needed for redox-dependent regulation of ATP synthase both in chloroplasts and in cyanobacteria

This study shows PGR5 regulates ATP synthase activity in photosynthetic organisms under fluctuating light through a thiol redox mechanism, contributing to photoprotection.

Kinetic parameter prediction using neural networks identifies limitations to C4 photosynthesis

C4TUNE predicts photosynthesis kinetic parameters from response curves, enabling efficient simulation and identification of limiting factors in maize genotypes.

Chemical modulation of chloroplast de- and re-differentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions

The paper shows that X57 modulates chloroplast transitions by inhibiting SAL1, leading to PAP accumulation and changes in gene expression that facilitate plastid de- and re-differentiation.

Stochastic spin selection in the mechanism of semiquinone formation at the ubiquinol oxidation Qo site of cytochrome bc1

The paper uses EPR spectroscopy to test two models of semiquinone formation in cytochrome bc1, finding support for the EMET model over the canonical EB model.

Characteristics of the C4 bundle sheath emerge in C3 rice after editing a plasma membrane proton ATPase

This study shows that editing the plasma membrane proton ATPase OSA3 in C3 rice alters bundle sheath cell length and number, mimicking C4 characteristics through acid growth mechanisms.

CO2 fixation mediated by the carbon concentrating mechanism enables a rapid response to nitrogen deprivation in cyanobacteria

This study shows that the CO2 concentrating mechanism in cyanobacteria enhances the speed of the nitrogen deprivation response by increasing Rubisco carboxylation rates.

Structure of Photosystem I-FCP from giant kelp uncovers drivers of antenna evolution across the red lineage

The paper determines the structure of Photosystem I-FCP from giant kelp and compares it to red-algal lineage structures to identify evolutionary drivers of antenna variation.

Structure of chlorophyll synthase in complex with the LHC-like protein HliD

The paper determines the structure of the ChlG2HliD2 complex using cryo-EM to elucidate chlorophyll synthesis and photoprotection mechanisms.

Chloroplast movements in siphonous macroalgae in response to high light and grazing

This study investigates chloroplast movements in two siphonous macroalgae under high light and grazing, finding that A. acetabulum responds with chloroplast aggregation while Bryopsis sp. does not.

Phenotypic heterogeneity in a batch culture of Chlamydomonas reinhardtii with different light tolerances.

The paper isolates two subpopulations from a genetically identical microalga culture and finds differences in light stress tolerance and photosynthetic performance.

Electron bifurcation arises from emergent features of multicofactor enzymes

This paper introduces the EMBER model of electron bifurcation, showing it explains experimental observations better than the canonical model by emphasizing emergent properties of redox cofactors.

Early-Diverging SQR Enzyme in Antarctic Gloeobacterales Indicates Sulfide Tolerance in Thylakoid-Lacking Cyanobacteria

The paper identifies an early-diverging sulfide quinone reductase in Antarctic Gloeobacterales, suggesting sulfide tolerance in thylakoid-lacking cyanobacteria and their adaptation to euxinic conditions.

Fitting photosynthetic carbon dioxide and irradiance response curves for C4 leaves

The paper updates a C4 photosynthesis model and provides fitting routines to estimate key parameters from CO2 and irradiance response curves using gas exchange measurements.

Uniform distribution of photosystems in dark-adapted Synechocystis cells

This paper uses cryo-ExM to visualize thylakoid membranes in dark-adapted Synechocystis cells and shows PSI, PSII, and PBS are highly colocalized, with no evidence for microdomains.

Structural basis of the two-photon photoactivation mechanism of orange carotenoid protein

The paper elucidates the structural basis of the two-photon photoactivation mechanism of orange carotenoid protein using a combination of structural and biochemical methods.

Coordinated cell and chloroplast growth and its perturbation by chloroplast DNA replication inhibition in green algae

This study examines how chloroplast DNA replication inhibition affects coordination between cell and chloroplast growth in green algae, revealing species-specific responses.

Photosynthesis regulation impacts carbon and nitrogen assimilation in the diazotrophic cyanobacterium Anabaena sp. PCC 7120

This study investigates how photosynthesis regulation affects carbon and nitrogen assimilation in Anabaena sp. PCC 7120 under varying environmental conditions.

SAGA1 and SAGA2 localize the starch sheath to the pyrenoid in Chlamydomonas reinhardtii

This paper identifies SAGA1 and SAGA2 as proteins necessary for localizing the starch sheath to the pyrenoid in Chlamydomonas reinhardtii, showing their redundant roles in starch sheath biogenesis.

Cyanobacteria form a procarboxysome-like structure in response to high CO2

The paper tracks redox states in cyanobacteria under different CO2 conditions, finding that procarboxysome-like structures form in high CO2, suggesting a more permeable shell is advantageous.

Improving tolerance to fluctuating light through adaptive laboratory evolution in the cyanobacterium Synechocystis

This study uses adaptive laboratory evolution to improve Synechocystis tolerance to fluctuating light, identifying mutations that enhance survival under both lethal and non-lethal conditions.

Endogenous short enhancer sequences increase expression of soybean and cowpea RUBP regeneration genes

The paper identifies short enhancer sequences in plant genomes that can increase gene expression, showing their potential for cisgenic promoter engineering.