Insect Resistance and Genetics

RNAi gene knockdown in the poultry red mite, Dermanyssus gallinae (De Geer 1778), a tool for functional genomics

This study identifies core RNAi pathway genes in D. gallinae and demonstrates effective dsRNA-mediated gene knockdown for functional genomics and control target validation.

Distinct roles of two RDL GABA-receptors in fipronil action in the diamondback moth (Plutella xylostella)

This study identifies two RDL GABA-receptors in diamondback moths, showing PxRDL1 mediates fipronil sensitivity while PxRDL2 contributes to resistance through differential expression and sensitivity.

Capturing the transcription factor interactome in response to sub-lethal insecticide exposure

This study uses dynamic Bayesian networks to analyze transcriptome time-course data from sub-lethal pyrethroid-exposed Anopheles coluzzii, identifying 44 transcription factors involved in pesticide response.

Incipient resistance to an effective pesticide results from genetic adaptation and the canalization of gene expression

The paper detects early genetic and transcriptomic changes in sea lamprey exposed to pesticide TFM, showing adaptation without full resistance.

Selective sweeps in a nutshell; the genomic footprint of rapid insecticide resistance evolution in a major insect pest of tree nuts

The paper examines genome-wide population genetics of insecticide resistance in navel orangeworms, finding a large selective sweep linked to DDT and pyrethroid resistance genes.

Genome-enabled insights into the biology of thrips as crop pests

The paper presents the first genome sequence for a thysanopteran insect, providing insights into its biology and evolution as a crop pest.

Comparison of in vitro and in planta toxicity of Vip3A for lepidopteran herbivores

This study compares the toxicity of Vip3A in vitro and in planta against lepidopteran pests, finding no correlation between the two and highlighting host plant adaptation effects.

A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G

The paper identifies a new kdr mutation, F1534L, in Ae. aegypti from Bengaluru, India, co-occurring with other kdr mutations and associated with permethrin resistance.

Multiplex PCR for simultaneous genotyping of kdr mutations V410L, V1,016I and F1,534C in Aedes aegypti (L.).

The paper develops a multiplex PCR method to detect three kdr mutations in Aedes aegypti and validates it against allele-specific PCR with high concordance.

Combination of kdr mutation and detoxification gene expression associated with high tolerance for permethrin in a resistant Aedes aegypti population

The paper identifies kdr mutations and upregulated detoxification genes as factors contributing to permethrin resistance in a Florida Aedes aegypti population.

Identifying candidate detoxification genes in the ecdysteroid kinase-like (EcKL) and cytochrome P450 gene families in Drosophila melanogaster by integrating evolutionary and transcriptomic data

The paper identifies candidate detoxification genes in Drosophila by integrating evolutionary and transcriptomic data, finding that EcKLs may function in detoxification and that many P450s are potential detoxification candidates.

Loss of pyrethroid resistance in newly established laboratory colonies of Aedes aegypti

The paper shows that pyrethroid resistance alleles in Aedes aegypti decline in frequency when pyrethroid pressure is removed, but the decline varies by strain.

Co-occurrence of kdr mutations V1016I and F1534C in pyrethroid-resistant Aedes aegypti (Diptera: Culicidae) populations from Costa Rica

High-depth resequencing reveals hybrid population and insecticide resistance characteristics of fall armyworm (Spodoptera frugiperda) invading China

This study uses high-depth resequencing to analyze genetic diversity and insecticide resistance in fall armyworm populations invading China, revealing hybrid origins and resistance patterns.

High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations

The paper develops a target capture sequencing method and analysis pipeline to genotype the full voltage-gated sodium channel gene in mosquitoes, enabling the discovery of novel insecticide resistance mutations.

Isolation of the Astacin-like metalloprotease coding gene (astl) and assessment of its insecticidal activity against Spodoptera littoralis and Sitophilus oryzae

The paper clones and expresses an astacin-like metalloprotease gene from a spider, tests its insecticidal activity against two agricultural pests, and evaluates the synergistic effect of combining it with a lectin.

Combining genetic crosses and pool targeted DNA-seq for untangling genomic variations associated with resistance to multiple insecticides in the dengue vector Aedes aegypti

This study combines genetic crosses with targeted DNA sequencing to identify resistance alleles in multi-resistant Aedes aegypti mosquitoes exposed to insecticides.

Oral RNAi of diap1 in a pest results in rapid reduction of crop damage

This study identifies diap1 as a more effective target for RNAi-based pest control due to its ability to rapidly reduce crop damage compared to conventional targets.

Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus

This study identifies genes associated with bendiocarb resistance in Ugandan Culex quinquefasciatus mosquitoes through transcriptomic analysis.

Development and Application of a Tri-allelic PCR Assay for Screening Vgsc-L1014F Kdr Mutations Associated with Pyrethroid and Organochlorine Resistance in the Mosquito Culex quinquefasciatus

The paper develops and validates an ETAS-PCR assay for detecting tri-allelic Vgsc-L1014F mutations in Culex quinquefasciatus, showing high correlation with established methods.

Cis and trans-acting variants contribute to survivorship in a naive Drosophila melanogaster population exposed to ryanoid insecticides

The paper identifies cis and trans-acting genetic variants affecting survivorship in Drosophila melanogaster exposed to ryanoid insecticides.

Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata

This study shows that silencing cathepsin L in aphids reduces their protein content and affects ladybug growth, highlighting the importance of prey nutritive value in RNAi-based pest control.

Window to the Fluorescence: The White Eye-Color Gene of Western Corn Rootworm, Diabrotica virgifera virgifera

The paper identifies and characterizes the Dvvw gene in western corn rootworm, showing its role in eye pigmentation and its utility in transgenic marker visualization.

Long-term population studies uncover the genome structure and genetic basis of xenobiotic and host plant adaptation in the herbivore Tetranychus urticae

The paper uses experimental evolution in spider mites to map genetic loci underlying pesticide resistance and host plant adaptation, revealing polygenic and locus-specific mechanisms.

Trans-generational transcriptomic response to natural variation in host plant toxicity and insecticides in a specialist insect.

The paper examines trans-generational transcriptomic responses of aphids to host plant toxicity and insecticides, finding increased transcriptional plasticity and changes in metabolic and regulatory genes.

Structural variants and selective sweep foci contribute to insecticide resistance in the Drosophila melanogaster genetic reference panel

The paper links insecticide resistance in Drosophila melanogaster to structural variants and selective sweep loci using genome-wide association studies.

Transcriptomic analysis reveals similarities in genetic activation of detoxification mechanisms resulting from imidacloprid and chlorothalonil exposure

The paper examines transcriptomic responses of Colorado potato beetles to imidacloprid and chlorothalonil, finding similar up-regulation of detoxification genes.

Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)

The paper quantifies permethrin resistance and kdr alleles in Florida Aedes aegypti and Ae. albopictus strains, finding widespread resistance and strong correlation between kdr alleles and resistance ratios.

A model species for agricultural pest genomics: the genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

The paper presents the genome of the Colorado potato beetle to study its rapid evolutionary changes, herbivory adaptations, and insecticide resistance mechanisms.

Partitioning The Roles Of Insect And Microbial Enzymes In The Metabolism Of The Insecticide Imidacloprid In Drosophila melanogaster

The paper investigates the roles of insect and microbial enzymes in imidacloprid metabolism in Drosophila melanogaster using genetic and mass spectrometry methods, finding microbial contributions to nitro-reduced metabolites and excretion of toxic products.