Viral Infectious Diseases and Gene Expression in Insects

This paper develops residual hybrid models combining mechanistic and machine learning to improve predictions of N-glycosylation and biopharmaceutical production, achieving significantly lower prediction errors.

The paper compares transcriptomic changes in CHO cells producing different recombinant mAbs, finding adaptive mRNA abundance changes linked to mAb production and cellular capacity.

The paper uses AI and machine learning to identify key targets in signaling pathways related to CHO cell death, aiming to optimize biopharmaceutical production.

The paper identifies a novel chimeric RNA formed between silkworm HDAC11 and BmCPV S4 transcripts that inhibits viral proliferation by altering histone modifications.

The paper identifies transcriptomic signatures that explain most of the variation in recombinant protein yield in CHO cells producing 2165 human secretome proteins.

The paper generates and tests short artificial introns to enhance transgene expression in mammalian cells, identifying 12 effective variants.

The paper demonstrates the use of CRISPR/Cas9 and PIG bombardment to edit the genome of Trichoplusia ni granulovirus in vivo, achieving reporter protein expression in infected larvae.

The paper presents genome assemblies and gene predictions for Sf21 and Tni cell lines using multi-omics data, enabling improved use in protein expression and genome engineering.

The paper describes modified E. coli strains that block native Tn7 attachment sites to reduce contamination from non-recombinant baculovirus production.

This study investigates the effects of elevated ammonia on genome instability in CHO cells, identifying de novo mutations and microsatellite instability as biomarkers for genome stability.

This study uses metabolic modeling to analyze amino acid requirements in CHO cells during recombinant protein production, finding that non-essential amino acids and D-serine significantly impact biomass and secretion.

The paper describes the expression and purification of the cytosolic domain of IRE1α in insect cells using a baculoviral system, achieving over 85% purity.