Brucella: diagnosis, epidemiology, treatment

Integrative reanalysis of public intracellular and vaccine-challenge transcriptomes, informed by single-cell and metabolomic atlases, refines antigen prioritisation for next-generation veterinary Brucella vaccines

The paper reanalyzes public transcriptomic and metabolomic data to prioritize antigens for improved Brucella vaccines by integrating single-cell and metabolomic atlases.

A genome-wide CRISPR screen defines host determinants of early Brucella infection in human macrophage-like cells

This study uses a CRISPR screen to identify host genes critical for early Brucella infection in human macrophage-like cells, revealing shared and pathogen-specific dependencies.

Structural Characterization of the Type IV Secretion System in Brucella melitensis for Virtual Screening-Based Therapeutic Targeting

This study models the T4SS structure of Brucella melitensis and identifies three FDA-approved drugs that could target the system for anti-virulence therapy.

Brucella proline racemase protein A targets Tpl2 to promote IL-10 secretion for establishment of chronic infection

This study identifies Brucella PrpA as a protein that activates the Tpl2-ERK pathway to induce IL-10 secretion in macrophages, promoting chronic infection and suggesting the prpA mutant as a potential vaccine.

Integrating multi-host modelling with empirical wildlife-livestock contacts reveals an essential population in a pathogen reservoir

The paper develops a multi-host model for brucellosis in the French Alps, identifying Alpine ibex and their environment as essential for pathogen maintenance and transmission to livestock.

Host GPCR-cAMP signaling balances Gαs and Gαi activity to control intracellular Brucella infection

Impact of Inactivation Methods on Biosafety and Antigen Reactivity of Brucella melitensis from the Perspective of Astral-DIA Proteomics Based on Antibody Immunoprecipitation Mass Spectrometry

This study evaluates inactivation methods for Brucella melitensis using Astral-DIA proteomics, finding that heat and formaldehyde affect antigenicity and protein expression differently across strains.

Immunogenicity and protective efficacy of a Brucella abortus L7/L12 DNA vaccine delivered via chitosan modified PLGA nanoparticles in mice

This study evaluates a DNA vaccine against Brucella abortus using chitosan-modified PLGA nanoparticles in mice, showing enhanced immunogenicity and protective efficacy compared to naked DNA.

Brucella abortus egresses from host cells in infective clumps through an actin-dependent mechanism

This study identifies an actin-dependent mechanism by which Brucella abortus exits host cells in infective clumps, forming extracellular vesicles that can reinfect neighboring cells.

Cell-to-cell transmission of Brucella abortus

This study identifies three modes of Brucella abortus exit from host cells, including cell-to-cell transmission, which may enhance immune evasion and persistence.

Persistent articular infection and host reactive response contribute to Brucella-induced spondyloarthritis in SKG mice

This study shows that Brucella-induced spondyloarthritis in SKG mice results from both persistent bacterial infection and host immune reactivity, with species-specific differences in disease progression.

Metabolomic Analysis of Murine Tissues Infected with Brucella melitensis

This study analyzes metabolomic changes in murine tissues infected with Brucella melitensis, finding significant metabolic alterations in reproductive tracts, spleens, and livers at two weeks post-infection.

Inside Out: the physiology of Brucella Vegetative and Intracellular Growth.

This study quantifies growth rates of Brucella species in vitro and intracellularly, revealing species-specific differences despite high genomic similarity.