bioluminescence and chemiluminescence research

Design and optimization of a cell-free atrazine biosensor

This paper designs a cell-free biosensor for atrazine detection using a metabolic pathway and a cyanuric acid biosensor, achieving faster and tunable detection compared to whole-cell sensors.

Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying

This paper develops a multiplex hextuple luciferase assay to simultaneously assess multiple cellular pathways and demonstrates its application in monitoring pathway responses to various treatments.

Detecting changes in the Caenorhabditis elegans intestinal environment using an engineered bacterial biosensor

The paper develops a bacterial biosensor system in C. elegans to detect environmental changes in the intestinal tract using an inducer.

Nanoluciferase-based Method for Detecting Gene Expression in C. elegans

This paper introduces a NanoLuc-based method for highly sensitive gene expression detection in C. elegans, showing it can detect signals at 400,000-fold over background in populations and from single worms.

Robust Normalization of Luciferase Reporter Data

The paper introduces a regression-based normalization method for Luciferase reporter data, showing it outperforms ratiometric normalization, especially at low transfection efficiency.

Firefly genomes illuminate parallel origins of bioluminescence in beetles

The paper generates genomes for three bioluminescent beetles to study the genetic basis of bioluminescence and finds at least two independent evolutionary origins of the trait.

Development of a GFP Fluorescent Bacterial Biosensor for the Detection and Quantification of Silver and Copper Ions

This paper develops two bacterial biosensors that detect and quantify silver and copper ions using GFP fluorescence, showing correlation between ion concentration and signal intensity.

Reconstructing Promoter Activity From Lux Bioluminescent Reporters

This paper develops a modeling approach to infer gene transcription levels from Lux bioluminescent data, addressing nonlinear dynamics that affect light output.