bioluminescence and chemiluminescence research

Development of a whole-cell biosensor for ethylene oxide and ethylene

The paper develops a whole-cell biosensor for ethylene oxide and ethylene using genetic parts from Mycobacterium, achieving detection limits of 0.2 μM for ethylene oxide and detecting ethylene from banana fruit.

ATP-Independent Water-Soluble Luciferins Enable Non-Invasive High-Speed Video-Rate Bioluminescence Imaging of Mice

The paper develops new luciferin substrates that enhance bioluminescence brightness and stability, enabling high-speed video-rate imaging of mice with improved sensitivity and non-invasive monitoring of calcium activity.

Finding Fluorescence: Utilizing community science to document novel biofluorescence occurrences and encourage community engagement in science

This paper introduces Finding Fluorescence, a community science platform that documents novel biofluorescence occurrences and highlights the ecological roles of fluorescence across species.

A Method for Optimizing Imaging Parameters to Record Neuronal and Cellular Activity at Depth with Bioluminescence

The paper develops an in vitro method to optimize imaging parameters for bioluminescence-based neuronal activity recording without animal use.

Engineering the soil bacterium Pseudomonas synxantha 2-79 into a ratiometric bioreporter for phosphorus limitation

The paper engineers a ratiometric bioreporter in Pseudomonas synxantha 2-79 to detect phosphorus limitation in soil, showing improved performance over native promoters and robustness across pH ranges.

Enhancing Luciferase Activity and Stability through Generative Modeling of Natural Enzyme Sequences

This study uses generative modeling and biochemistry to improve luciferase activity and stability by engineering its active center and scaffold, achieving a 50% success rate for single mutants.

A quantitative autonomous bioluminescence reporter system with a wide dynamic range for Plant Synthetic Biology

The paper develops a high-throughput, quantitative bioluminescence reporter system for plant synthetic biology with improved dynamic range and application in synthetic gene circuit optimization.

LucFlow: A method to measure Luciferase reporter expression in single cells

LucFlow measures Luciferase reporter expression in single cells with higher accuracy than bulk methods by excluding dead cells.

Bioluminescent Genetically Encoded Glutamate Indicator for Molecular Imaging of Neuronal Activity

The paper develops a bioluminescent genetically encoded glutamate indicator for in vivo neuronal activity monitoring without requiring external light sources.

Structural characterization of a novel luciferase-like-monooxygenase from Pseudomonas meliae an in silico approach

This study models and analyzes a luciferase-like monooxygenase from Pseudomonas meliae, identifying mutations that prevent bioluminescence and suggesting ways to restore its function.

Expanding luciferase reporter systems for cell-free protein expression

This paper expands luciferase reporter systems for cell-free protein expression by introducing two orthogonal luciferases and a substrate regeneration pathway.

A modular platform for bioluminescent RNA tracking

This paper introduces a bioluminescent platform for tracking RNA in vivo, demonstrating single-copy detection and robust imaging in cells and live animals.

Semi-automated analysis of an optical ATP indicator in neurons

This paper develops a semi-automated pipeline using machine learning to improve ATP measurement accuracy in nerve terminals from bioluminescence images.

Non-destructive chemical sensing within bulk soil using 1000 biosensors per gram of matrix

This study demonstrates that gas-reporting biosensors can detect gene expression in environmental soil matrices with minimal perturbation by optimizing hydration and biosensor titer.

Real-time luminescence enables continuous drug-response analysis in adherent and suspension cell lines

This paper introduces a method using real-time luminescence to estimate drug response in cell lines, avoiding the need for cell counting and fluorescent labeling.

LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters

LuminoCell is an affordable, open-source luminometer platform for real-time monitoring of in-cell luciferase activity in various biological assays.

Re-engineering of CUP1 promoter and Cup2/Ace1 transactivator to convert Saccharomyces cerevisiae into a whole-cell eukaryotic biosensor capable of detecting 10 nM of bioavailable copper

The paper develops a whole-cell eukaryotic copper biosensor in yeast with improved sensitivity and specificity for detecting bioavailable copper at low concentrations.

A dual-purpose real-time indicator and transcriptional integrator for calcium detection in living cells

This paper introduces LuCID, a tool that detects calcium in real-time and converts it into stable gene expression, with applications beyond calcium signaling.

A luciferase prosubstrate and a red bioluminescent calcium indicator to image neuronal activity

This paper develops a bioluminescent calcium indicator and prosubstrate for non-invasive imaging of neuronal activity in awake mice.

Draft genome of the Brazilian railroad worm Phrixothrix hirtus E.Olivier (Phengodidae: Coleoptera)

The paper presents the first draft genome assembly of Phrixothrix hirtus, revealing gene family expansions and bioluminescence-related genes shared with Elateroidea.

An Autonomous Molecular Bioluminescent Reporter (AMBER) for voltage imaging in freely moving animals

The paper introduces AMBER, a genetically encoded bioluminescent voltage indicator that does not require exogenous substrate, enabling voltage imaging in freely moving animals.

PlotXpress, a webtool for normalization and visualization of reporter expression data

PlotXpress is a webtool that normalizes and visualizes reporter expression data using internal controls and provides standardized, publication-grade plots.

Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters

The paper develops a Golden Gate assembly system for constructing Escherichia coli bioreporters that detect mercury and arsenic under aerobic and anaerobic conditions.

A ratiometric dual color luciferase reporter for fast characterization of transcriptional regulatory elements

The paper develops a dual-color luciferase reporter system for faster and more consistent characterization of transcriptional regulatory elements in plants.

Flash properties of Gaussia Luciferase are the result of covalent inhibition after a limited number of cycles

The paper investigates the 'flash' kinetics of Gaussia Luciferase, showing that the decay in light signal is due to irreversible enzyme inactivation caused by covalent substrate binding.

Mycena genomes resolve the evolution of fungal bioluminescence

The paper sequenced genomes of five bioluminescent Mycena fungi to study the evolution of luciferase clusters and bioluminescence, finding that the trait originated in a common ancestor and was lost in some lineages.

First report of an insilico study of Luciferase in Mycobacterium sp. EPa45

This paper reports an in silico analysis of luciferase in Mycobacterium sp. EPa45, comparing its physiochemical, structural, and phylogenetic characteristics to other mycobacterial and bacterial strains.

De novo design of modular and tunable allosteric biosensors

The paper designs modular biosensors using de novo protein switches that detect various analytes with high sensitivity and modularity.

Laboratory culture of the California Sea Firefly Vargula tsujii (Ostracoda: Cypridinidae): Developing a model system for the evolution of marine bioluminescence

This paper reports the first complete life cycle and mitochondrial genome of the luminous ostracod Vargula tsujii, enabling future studies on bioluminescence biosynthesis and genomic manipulation.

Ratiometric bioluminescent sensors towards in vivo imaging of bacterial signaling

This paper develops a ratiometric bioluminescent biosensor to image cyclic di-GMP levels in bacteria during host infection.